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goat anti mouse trem 1 ab  (R&D Systems)


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    Structured Review

    R&D Systems goat anti mouse trem 1 ab
    Goat Anti Mouse Trem 1 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+trem+1+ab/Mouse+TREM-1+Antibody/pmc07141396-545-12-18
    Average 94 stars, based on 12 article reviews
    goat anti mouse trem 1 ab - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Injection:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Staining:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Confocal Microscopy:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Microscopy:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Software:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Fluorescence:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Immunoprecipitation:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Western Blot:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Expressing:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Comparison:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Transfection:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Förster Resonance Energy Transfer:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Activation Assay:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Saline:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Sequencing:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Sterility:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Incubation:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).

    Derivative Assay:

    Article Title: Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Article Snippet: After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories).. Antibodies used were as follows: rabbit anti-mouse CIRP Ab (catalog 10209-2-AP; ProteinTech), goat anti-mouse TREM-1 Ab (catalog AF1187; R&D Systems, Bio-Techne), goat anti-CD11b Ab (catalog MBS420973; MyBioSource), Cy5-conjugated AffiniPure donkey anti–goat IgG (code 705-175-147; Jackson ImmunoResearch Laboratories) and Cy3-conjugated AffiniPure F(ab′) 2 donkey anti–rabbit IgG (code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.Confocal microscopy images were obtained using a Zeiss LSM880 confocal microscope equipped with a 63× objective.

    Article Title: Extracellular CIRP as a Novel Endogenous TREM-1 Ligand to Fuel Inflammation
    Article Snippet: After washing with PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA).PBS, cells were incubated in the dark with diluted secondary Abs for 1 h. After an additional washing step, slides were mounted immediately on Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). ... Antibodies used were as follows: rabbit anti-mouse CIRP Ab (Catalog 10209-2-AP; ProteinTech, Rosemont, IL), goat anti-mouse TREM-1 Ab (Catalog AF1187; R&D Systems), goat anti-CD11b Ab (Catalog MBS420973MyBioSource, San Diego, CA), Cy5-conjugated AffiniPure donkey anti-goat IgG (Code 705-175-147; Jackson ImmunoResearch Laboratories, West Grove, PA) and Cy3-conjugated AffiniPure F(ab’)2 fragment donkey anti-rabbit IgG (Code 711-166-152; Jackson ImmunoResearch Laboratories).. Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).Confocal microscopy images were obtained at using a Zeiss LSM880 confocal microscope equipped with a 63× objective (Zeiss, Oberkochen, Germ).



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